The immune cells are postulated to exert a pivotal role in the development of fibrotic skin disease. Single-cell RNA sequencing has been used to explore the composition and functionality of immune ...

Methods PBMCs from 32 subjects (ANA-, ANA+, ILE and SLE) were sorted with Nanocellect Wolf microfluidic flow cytometer to remove dead and dying cells. Viable isolated single cells were used to do a ...

Single-cell RNA sequencing (scRNA-seq) research of mouse and human CD8 + T cells revealed a surprising population of distinct age-related CD8 + T cells (5). Similarly, scRNA-seq from aged and young ...

You can also normlize the data through UMI_counts data in your way. #4： The supplemental discussion includes two parts: (i) The Expression Characteristics of Ribosome-associated Genes and RNA-binding ...

All code required to reproduce the main figures of the paper “The heterogeneity of human CD127+ innate lymphoid cells revealed by single-cell RNA sequencing” Björklund, Forkel et al. The first part of ...

Single nucleus RNA sequencing (snRNA-seq), an alternative to single cell RNA sequencing (scRNA-seq), encounters technical challenges in obtaining high-quality nuclei and RNA, persistently hindering ...

163.com Objectives The heterogeneity of meniscus cells and the mechanism of meniscus degeneration is not well understood. Here, single-cell RNA sequencing (scRNA-seq) was used to identify various ...

The Single Cell & Transcriptomics Core is on the leading edge of single cell research at Johns Hopkins. We offer three different platforms with the ability to sequence RNA, DNA and protein on a single ...

We offer a range of services, including single cell RNA sequencing and spatial transcriptomics. Our facility is equipped with state-of-art instruments, including the 10X Genomics Chromium X which ...

Here, we report a systematic transcriptional atlas to delineate molecular and cellular heterogeneity in GA using single-cell RNA sequencing (scRNA-seq). Design We performed unbiased transcriptome-wide ...