2009年10月13日 · These findings provide unique insights into the genesis of red cell membrane function during erythroblast differentiation and also offer a means of defining stage-specific defects in erythroid maturation in inherited and acquired red cell disorders and in bone marrow failure syndromes.

ERYTHROID DIFFERENTIATION WITHIN THE ADULT HEMATOPOIETIC HIERARCHY. Erythroid cells are derived from HSCs, but the differentiation path they follow is still a matter of debate (Buza-Vidas et al. 2007). Immature progenitors are capable of giving rise to multilineage colonies, but whether there is a fixed order to cell fate decisions (i.e., at ...

2006年1月19日 · Here we report the existence of erythroid differentiation factors in erythroid cytoplasm. Purification of these factors from cultured Friend virus anaemia (FVA)‐infected mouse splenic erythroblasts was carried out using isoelectrophoresis and high performance of liquid chromatograpy techniques.

2019年11月4日 · Expanded erythroblast cultures could be differentiated to CD71 dim CD235a + CD44 + CD117 − DRAQ5 − RBC in 12 days. More than 90% of the cells enucleated and expressed adult hemoglobin as well as the correct blood group antigens. Deformability and oxygen-binding capacity of cultured RBC was comparable to in vivo reticulocytes.

Human cRBCs can be generated from the terminal differentiation of proliferating erythroblasts derived from hematopoietic stem/progenitor cells or erythroid precursors in peripheral blood mononuclear cells.

2024年12月4日 · Understanding how mechanical stress affects erythropoiesis is crucial to produce transfusable erythrocytes in fluid-turbulent bioreactors. We investigated the effects of shear-stress on...

In the present study, we found that a newly developed cell culture system (human plasma‐like medium), mimicking the metabolic profile of human plasma, enhances erythroblast proliferation and increases cRBC yield.

2022年8月5日 · This study shows that erythroblast expansion cultures can withstand agitation speeds of up to 600 rpm (tip speed = 880 mm/s), without negatively affecting growth, viability, or the differentiation stage of the cells.

2022年6月21日 · Here we show, using mouse models of Cdh1 deletion, that APC/C-Cdh1 activity is required for efficient terminal erythroid differentiation during fetal development as well as postnatally....

These findings provide unique insights into the genesis of red cell membrane function during erythroblast differentiation and also offer a means of defining stage-specific defects in erythroid maturation in inherited and acquired red cell disorders and in bone marrow failure syndromes.